Characterization of Npas4 and heterodimer DNA binding in stimulated and silenced rat neurons

We evaluated genome-wide DNA binding of NPAS4, ARNT1, ARNT2, H3K27ac, and RNAPII by chromatin immunoprecipitation sequencing (ChIP-seq) in mature primary hippocampal neurons (rat, DIV 28) that were pharmacologically silenced (“–”; 24 hours in TTX, CPP, and NBQX) or silenced and then treated with PTX for two hours (“+”) to trigger the production of AP- and EPSP-induced NPAS4. Overall design: ChIP-seq for Npas4, Arnt1, Arnt2, H3K27ac, and RNAPII were performed in stimulated and silenced cultured rat neurons, with 2 biological replicated of each ChIP.