Resistance gene-dependent responses in rice to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae

TITLE: Transcriptional profiling of Rgene-mediated responses in rice PROJECT DESCRIPTION: The dominant gene Xa7 and the recessive gene xa5 of rice confer resistance to several races of the bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). To reveal the modes of action and the defense responses these genes initiate, we decided to obtain the global transcriptional profiles of the rice cultivars IRBB7, IRBB5 (which harbor Xa7 and xa5, respectively) and IR24 undergoing early infection by the Xoo Race 2 strain PXO86. Both IRBB7 and IRBB5 are resistant to PXO86 (which carry the corresponding avirulence genes avrXa7 and avrxa5), whereas IR24 is susceptible. We inoculated by vacuum infiltration the three rice cultivars ten days after seed germination (or 2 weeks after sowing) and collected inoculated tissue at 5 different timepoints within the first day after inoculation. The transcriptional profiles obtained will provide valuable insight into the similarities and differences between incompatible interactions mediated by a dominant and a recessive Rgene, in comparison to a compatible interaction. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, David O Nino-Liu. The equivalent experiment is OS4 at PLEXdb.] genotype: IR24 - time: 0 hai(3-replications); genotype: IR24 - time: 3 hai(3-replications); genotype: IR24 - time: 6 hai(3-replications); genotype: IR24 - time: 12 hai(3-replications); genotype: IR24 - time: 24 hai(3-replications); genotype: IRBB5 - time: 0 hai(3-replications); genotype: IRBB5 - time: 3 hai(3-replications); genotype: IRBB5 - time: 6 hai(3-replications); genotype: IRBB5 - time: 12 hai(3-replications); genotype: IRBB5 - time: 24 hai(3-replications); genotype: IRBB7 - time: 0 hai(3-replications); genotype: IRBB7 - time: 3 hai(3-replications); genotype: IRBB7 - time: 6 hai(3-replications); genotype: IRBB7 - time: 12 hai(3-replications); genotype: IRBB7 - time: 24 hai(3-replications)