L. lactis ?lmrCD and cholate-adapted L. lactis ?lmrCDR. Lactococcus lactis

Transcriptome profiling of a L. lactis strain lacking the MDR transporter genes lmrCD and the same strain adapted to cholate was performed. As compared to the parental strain, the cholate-adapted strain showed 124 genes that were significantly differentially expressed more than 1.8-fold . These genes could be grouped into four major functional classes: i.e., genes involved in (i) cell envelope biogenesis; (ii) stress response and molecular chaperones; (iii) general metabolism and house-keeping function; and (iv) the chromosomally embedded sex-factor. Keywords: Mutant versus wild type comparison, stress response, cholate Overall design: 1. WT1 L. lactis ?lmrCD (labeled with Cy3) 2. WT2 L. lactis ?lmrCD (labeled with Cy5) 3. WT4 L. lactis ?lmrCD (labeled with Cy3) 4. WT5 L. lactis ?lmrCD (labeled with Cy5) 5. M1 L. lactis ?lmrCD(R) (labeled with Cy5) 6. M2 L. lactis ?lmrCD(R)(labeled with Cy3) 7. M4 L. lactis ?lmrCD(R) (labeled with Cy5) 8. M5 L. lactis ?lmrCD(R) (labeled with Cy3) Hybridization schema: 1+5 (Slide 176631) 3+7 (Slide 176632) 2+8 (Slide 176633) 4+6 (Slide 176634)