The effect of the MYH9 in endothelial cells on metabolism

This document focuses on exploring the specific impact of MYH9 on the metabolic processes of vascular endothelial cells. To achieve this, a well-designed experimental approach was adopted: human umbilical vein endothelial cells (HUVECs) were subjected to different treatments using adenoviruses, including MYH9 knockdown (MYH9_KD), knockdown control (MYH9_KDC), overexpression (MYH9_OE), and overexpression control (MYH9_OEC).
Subsequently, untargeted metabolomics analysis based on ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) was employed to systematically profile the metabolic changes in these treated cells. Through this comprehensive analysis, a total of 152 differential metabolites were identified, which exhibit distinct expression patterns across the different treatment groups. These differential metabolites are closely associated with several key metabolic pathways, with sulfur metabolism and glycerophospholipid metabolism being particularly prominent.
To further validate the reliability of the findings, seven core metabolites were selected for verification in the overexpression model, and their expression changes were confirmed, strengthening the credibility of the results. Overall, this study not only identifies potential biomarkers that may be related to MYH9-mediated vascular endothelial cell metabolism but also provides a solid mechanistic basis for understanding the role of MYH9 in the pathogenesis of vascular diseases, which is of great significance for the development of novel diagnostic and therapeutic strategies for MYH9-related vascular disorders.