Total RNA sequencing of a time course treatment of human embryonic stem cells with CHIR99021, recombinant Wnt3a, and a time course activation of constitutively active beta-catenin expression

Here we analysed the transcriptomic response of human embryonic stem cell line H9 to sustained activation of the canonical Wnt pathway by eiother treating cells with CHIR99021 or recombinant Wnt3a or inducing the overexpression of constitutively active beta-catenin mutant hESC cell line H9 was treated with 10 µM CHIR99021 or 240 ng/ml of rWnt3a. In parallel, H9 line with stable integration of doxycycline-regulatable deltaN90 mutant of beta-catenin was treated with 1 µg/ml of Doxycycline. Cells were collected at time points 0 hours (undifferentiated cells), 8 hours, 16 hours, 24 hours, 48 hours and 72 hours (additionally 96 and 120 hours for rWnt3a treatment) and subjected to total RNA sequencing using Illumina platform.