Expression data of intestinal polyps and intestinal normal tissue from Ubc9+/+ and Ubc9+/- Villin-CreERT2;Apcf/+ mice 12 weeks after 4-OHT treatment

Most human cancers present hyperactivated sumoylation, and cancer cell lines are usually highly sensitive to the lack of it, supporting potential application of sumoylation chemical inhibitors in cancer therapy. Here, we explored the impact of hyposumoylation (Ubc9 haploinsufficiency) on cancer development in mice using Apc loss-driven intestinal tumorigenesis model. We used microarrays to compare the global program of gene expression in intestinal polyps and normal tissue from mice homozygous (+/+) and heterozygous (+/-) for Ubc9, which encodes the unique SUMO E2-conjugating enzyme. This analysis was performed in the context of a conditional ablation of tumor suppressor Apc all along the crypt-villus axis through expresion of Cre recombinase driven by the intestinal specific promoter Villin Instestinal adenomatous polyps (≥0.5 mm in diameter) and intestinal normal tisues were harvested from washed and londitudinally openned intestines obtained 12 weeks after 4-OHT treatment of 8-12 weeks-old mice. RNA was extracted using Trizol protocol and high-quality RNA was used for hybridizationand to obtain the trasncriptome by using GeneChip MoGene 2.0 ST arrays. 2 polyps and 2 normal samples were analysed per mice. 3 female mice were used per genotype.