The protein U5-52K is required for T cell homeostasis by maintaining splicing efficiency

U5-52K is a nuclear protein present in the U5 snRNP spliceosome subunits and it is essential for the mouse embryo development. In fact its absence blocks embryo at the developmental stage at E 10.5 with subsequence reasbsorbtion. Therefore, we used a CD4-Cre-knockout strategy to abrogate U5-52K in T cells, allowing us to delineate the consequences of splicing machinery interferences with T cell homeostasis. Several Differentially Spliced Variants and Differentially Expressed Genes were detected in T cells lacking U5-52K indicating its involvement in gene transcription regulation. The RNA extracted from CD4+ isolated T cells from spleen and lymph nodes from three cKO mice (U5-52Kf/f CD4-Cretg) and three control mice (Control: U5-52Kwt/f CD4-Cretg and U5-52Kf/f CD4-Cre-) were sequenced using RNA-seq technology.