RNA-seq of the copepod Pseudocalanus newmani using poly(A) selection approach

To investigate the physiological changes in copepods with viral loads, RNA-seq data using poly(A) selection approach was obtained for the copepod Pseudocalanus newmani. Zooplankton samples were collected using NORPAC net with a 335-µm mesh size at the Okhotsk Tower, located 1 km off the coast of Mombetsu, northeastern Hokkaido, Japan. Adult female P. newmani were preserved in RNAlater, and RNA was extracted from each individual using AllPrep DNA/RNA Micro Kit (Qiagen). RNA quality was assessed using the 4200 TapeStation (Agilent Technologies), and RNA concentration was measured using a Qubit 3.0 Fluorometer (Life Technologies). Total RNA was purified using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), and the sequencing libraries were constructed using the TaKaRa SMART-Seq Stranded Kit (TaKaRa). We obtained 2 � 150 bp paired-end sequences by sequencing on the Illumina HiSeq platform.