Data for Galaxy CLIP-Seq Training Material

The eCLIP data provided here is a subset of the eCLIP data of RBFOX2 from a study published by Nostrand et al. (2016, http://dx.doi.org/10.1038/nmeth.3810). The dataset contains the first biological replicate of RBFOX2 CLIP-seq and the input control experiment (*fastq files). The data was changed and downsampled to reduce data processing time, thus the datasets does not correspond to the original data pulled from Nostrand et al. (2016, http://dx.doi.org/10.1038/nmeth.3810). Also included is a text file (.txt) encompassing the chromosome sizes of hg19 and hg38 obtained from UCSC (http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/hg19.chrom.sizes, http://hgdownload.cse.ucsc.edu/goldenPath/hg38/bigZips/hg38.chrom.sizes) and a genome annotation for hg19 (.gtf) taken from Ensembl (http://ftp.ensemblorg.ebi.ac.uk/pub/release-74/gtf/homo_sapiens/) and for hg38 taken from the Galaxy libraries (https://usegalaxy.eu/library/list#folders/F30cab321d898d2fb/datasets/9ba790aa79c9cf23). The data is used for a galaxy training course about CLIP-Seq data analysis.本数据集所提供的eCLIP（enhanced Cross-Linking Immunoprecipitation）数据，为Nostrand等人2016年发表的研究中RBFOX2蛋白eCLIP数据的子集（原文链接：http://dx.doi.org/10.1038/nmeth.3810）。该数据集包含RBFOX2 CLIP-seq（交联免疫沉淀测序）的首次生物学重复样本及其输入对照实验的fastq格式文件。为缩减数据处理时长，我们对原始数据进行了修改与降采样操作，因此本数据集与Nostrand等人2016年研究中的原始数据并不一致（原文链接：http://dx.doi.org/10.1038/nmeth.3810）。此外，本数据集还包含一份文本文件（.txt格式），其中收录了从UCSC数据库下载的hg19及hg38版本人类参考基因组的染色体大小信息（相关链接：http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/hg19.chrom.sizes、http://hgdownload.cse.ucsc.edu/goldenPath/hg38/bigZips/hg38.chrom.sizes）；同时包含hg19版本的基因组注释文件（.gtf格式，源自Ensembl数据库，链接：http://ftp.ensemblorg.ebi.ac.uk/pub/release-74/gtf/homo_sapiens/），以及hg38版本的基因组注释文件（.gtf格式，源自Galaxy数据库，链接：https://usegalaxy.eu/library/list#folders/F30cab321d898d2fb/datasets/9ba790aa79c9cf23）。本数据集用于一门关于CLIP-seq数据分析的Galaxy培训课程。