Label free comparative proteome analysis of Arabidopsis lipid droplets from 6 week old leaves

Lipid droplets from 6 week old Arabidopsis leaves were purified on sucrose gradient. The abundance of the proteins in the lipid droplet fraction was established by label free and compared to soluble, membrane and plastoglobule fractions, and total leaf extract in order to identify the proteins specifically enriched in lipid droplets. Three independent biological replicates, from three independent cultures were performed. For each biological replicates, three preparations of lipid droplets (and other fractions) within each of the three independent cultures were obtained.