Endothelial TGFß signaling drives vascular inflammation and atherosclerosis [scRNA-Seq]

Endothelial TGFß signaling is one of the primary drivers of atherosclerosis-associated vascular inflammation.  Inhibition of endothelial TGFß signaling in hyperlipidemic mice reduces vessel wall inflammation and vascular permeability and leads to arrest of disease progression and regression of established lesions. We performed scRNAseq method to examine endothelial cell gene expression profile using Apoe and EC specific TGFbR1/2 KO in Apoe background mice. Overall design: Using scRNAseq to compare the gene expression profiles of endothelial cells beteewn ApoE mice and EC specific TGFbR1/2 KO Apoe mice fed with high fat diet