Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.65A resolution

Crystal structure of the A2503-C2,C8-dimethylated Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.65A resolution Descriptor: 16S Ribosomal RNA, 23S Ribosomal RNA, 30S ribosomal protein S10, ... Authors: Aleksandrova, E.V, Wu, K.J.Y, Tresco, B.I.C, Syroegin, E.A, Killeavy, E.E, Balasanyants, S.M, Svetlov, M.S, Gregory, S.T, Atkinson, G.C, Myers, A.G, Polikanov, Y.S. Deposit date: 2023-02-03 Release date: 2023-12-27 Last modified: 2026-03-04 Method: X-RAY DIFFRACTION (2.65 Å) Cite: Structural basis of Cfr-mediated antimicrobial resistance and mechanisms to evade it. Nat.Chem.Biol., 20, 2024

分辨率为2.65埃的A2503-C2、C8二甲基化嗜热栖热菌70S核糖体晶体结构，该复合物结合了泰乐菌素（tylosin）、信使RNA（mRNA）、氨酰化A位点苯丙氨酸tRNA（Phe-tRNA^Phe）、氨酰化P位点甲酰甲硫氨酸tRNA（fMet-tRNA^met）以及脱酰基E位点苯丙氨酸tRNA（tRNA^Phe）。
描述项：16S核糖体RNA（16S Ribosomal RNA）、23S核糖体RNA（23S Ribosomal RNA）、30S核糖体蛋白S10……
作者：Aleksandrova, E.V、Wu, K.J.Y、Tresco, B.I.C、Syroegin, E.A、Killeavy, E.E、Balasanyants, S.M、Svetlov, M.S、Gregory, S.T、Atkinson, G.C、Myers, A.G、Polikanov, Y.S.
提交日期：2023-02-03
发布日期：2023-12-27
最后修改日期：2026-03-04
实验方法：X射线衍射（X-RAY DIFFRACTION，分辨率2.65埃）
引用文献：《Cfr介导的抗菌耐药性结构基础及其逃逸机制》，Nat.Chem.Biol., 20, 2024