Porcine Ovarian Cortex RNA-Sequencing

Delayed attainment of puberty is a major reason for removal of replacement gilts from the herd. To investigate the physiological factors underlying delayed puberty in gilts, transcriptomic data from ovarian cortex were obtained from age-matched delayed puberty gilts and normal cyclic control gilts at follicular phase and luteal phase of the estrous cycle. A three-way gene x individual x tissue clustering analysis was performed using tensor decomposition to obtain gene expression modules. Additionally, differences in gene expression in the hypothalamic-pituitary-ovary axis was analyzed between delayed puberty and cyclic gilts to gain understanding of how the tissues may differ between gilts that are of similar ages but at different levels of sexual development.

青春期延迟是剔除后备母猪的主要原因。为探究后备母猪青春期延迟的生理因素，本研究从年龄匹配的青春期延迟母猪和正常周期性控制母猪的卵巢皮质中获取了转录组数据，这些数据分别来自于发情周期的卵泡期和黄体期。通过张量分解方法执行了基因 x 个人 x 组织的三向聚类分析，以获得基因表达模块。此外，分析了青春期延迟母猪与周期性母猪在下丘脑-垂体-卵巢轴基因表达差异，旨在理解在性发育水平不同但年龄相近的母猪之间，组织可能存在的差异。