Combined metabolomics and transcriptomics reveal the secondary metabolite networks in different growth stages of Bletilla striata (Thunb.) Reichb.f.

The tubers of B. striata were collected during the flowering period, fruiting period, and harvest period to determine the total polysaccharide content using the phenol sulfuric acid method. The changes in secondary metabolites in the tubers at the three stages were analyzed by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS), and further exploration of their biosynthetic pathways was conducted through transcriptomics.