Anisakis simplex Raw sequence reads. Transcriptomic profile

This experimental study aims to investigate the gene expression plasticity of free-living infective third-stage larvae (L3) of Anisakis simplex in response to extreme thermal stress, potentially linked to the increasing occurrence of climate change-induced marine heatwaves. Using an RNA-seq approach, the molecular transcriptomic responses of L3 larvae were analyzed under both normal (15 C) and extreme (28 C) heating aquarium environmental stress conditions. Data were collected from 90 viable L3 in good condition, sampled from viscera of fresh European hake (Merluccius merluccius), and subjected to a seawater survival assay simulating the most extreme ocean warming event currently recorded within the Spanish Marine Demarcation. A bioinformatic pipeline involving read alignment (STAR), differential expression (DESeq2), and gene ontology enrichment was applied. The analysis revealed that larvae remained viable for up to 60 days at 15 C but only a week at 28 C. Transcriptomic data showed from 241 to 244 DEGs depending on each comparison, with a progressive downregulation of cuticle-related genes, a strong upregulation of peptidases, and coordinated neural modulation, with reduced ion channel activity and enhanced expression of key neurotransmission-related genes. Additionally, there was strong upregulation of genes involved in glycogen degradation and galactose metabolism, along with consistent downregulation of glycolysis-related genes. The transcriptomic patterns described above indicate that short-term marine heatwave conditions (28 C) severely compromise viability of free L3 of A. simplex, which clearly prioritize survival processes while suppressing developmental pathways. These findings suggest that the more intense, longer-lasting, and widespread marine heatwaves observed along the Spanish marine demarcation over the last 15 years may influence parasite persistence and transmission of infective stages to paratenic fish hosts. This highlights the One Health significance of such adaptive response, particularly regarding parasite biogeographical dispersion and associated zoonotic risk.