16s rRNA from maize rhizosphere soil bacterial

In order to study the influence of γ-PGA on bacterial community diversity under drought stress, the relative abundance and diversity of maize rhizospheric soil bacteria were analyzed by high-throughput sequencing of 16S rRNA.Amplification and High-throughput sequencing of 16s rRNA from maize rhizosphere soil bacterial were performed as described by Wang et al.(Wang et al.). The primers of V4 region of bacterial 16S rRNA were 338F (5’-ACTCCTACGGGAGGCAGCA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). High-throughput sequencing was performed by Illumina Hiseq 2000 (Illumina Inc., San Diego, USA).