k-mer matrix Aegilops tauschii diversity panel (Open wild wheat consortium phase II) Part 1/3

Wild wheat relatives of bread wheat represent genetic diversity that can be used for wheat crop improvement. We generated a k-mer presence/absence matrix of over 920 accessions of the wild wheat Aegilops tauschii, the donor of the bread wheat D genome. This dataset was generated under the aegis of Phase II of the Open Wild Wheat Consortium (www.openwildwheat.org)., The full methods are available in the related publication., , # *k*-mer matrix Aegilops tauschii diversity panel (Open Wild Wheat Consortium Phase II) PART 1/3

[https://doi.org/10.5061/dryad.p5hqbzkvx](https://doi.org/10.5061/dryad.p5hqbzkvx)

## Description of the data and file structure

*k*-mer presence/absence matrix for the *Aegilops tauschii* diversity panel of over 920 accessions, including genetically redundant accessions.

**Resequencing of the *Ae. tauschii* accessions:** We generated short-read whole genome sequencing data for 350 *Ae. tauschii *accessions.  PCR-free paired-end libraries were constructed and sequenced on an Illumina Novaseq 6000 instrument, yielding a median 8.3-fold coverage per sample (ranging from 5.87 to 16.86-fold).

***k*****-mer matrix generation**. We developed and implemented an optimised *k*-mer matrix workflow to generate a presence/absence *k*-mer matrix for large diversity panels (https://github.com/githubcbrc/KGWASMatrix). We counted *k*-mers (k = 51) in raw sequencing data for 350 accessions generated ...