Seasonal genotypic and phenotypic differentiation of a cosmopolitan freshwater diatom

Most ecosystems are characterized by seasonality, which, through biotic and abiotic changes, influences species biomass dynamics. Recent studies have shown that ecologically important traits can evolve rapidly in response to environmental changes, resulting in eco-evolutionary dynamics with consequences for population and community dynamics. Evidence for seasonal effects on intraspecific variation is still scarce, and understanding eco-evolutionary dynamics in the presence of seasonal fluctuations remains a major challenge. Following the phytoplankton spring bloom in Lake Constance, we investigated how seasonal changes influence the intraspecific diversity of Asterionella formosa both at genotypic and phenotypic levels. We found a moderate degree of genetic and phenotypic differentiation characterizing the Asterionella population, explained by a clustering of the isolates into early and late spring according to lake thermal stratification. Yet, most traits related to environmental ..., , , # Data from: Seasonal genotypic and phenotypic differentiation of a cosmopolitan freshwater diatom

[https://doi.org/10.5061/dryad.k6djh9wfc](https://doi.org/10.5061/dryad.k6djh9wfc)

## Description of the data and file structure

This repository is associated with Cristini, D., Kelly, J. B., Mahler, P., Schleheck, D., Lerner, H., & Becks, L. (2025). Seasonal genotypic and phenotypic differentiation of a cosmopolitan freshwater diatom.

The data are collected in five different folders: Lake_data.zip, Genotypic_data.zip, Trait_data.zip, IXM_protocols.zip and 18S_rDNA_data.zip.

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**Lake_data.zip:**

The folder contains the data collected in the field (Lake Constance) and reported in Figure 1 of the manuscript.

* \"Temperature.csv\": the file contains the temperature values collected in Lake Constance during the sampling season and used to represent the temperature profiles reported in Figure 1a;
* \"Phyto_biov+DeltaT.csv\": the file contains the temperature and phytoplankton data used..., ,