The effect of CLEC16A in Dendritic Cells (Single Cells)

CLEC16A were conditionally knocked out in Dendritic Cells, and the dendritic cells were sorted out using FACS and 10X single cell RNA-Seq protocol was used to profiled the transcriptome of individual cells. Overall design: For single cell RNA-sequencing, Spleen Dendritic cells from n=3 Control EAE and n=3 CLEC16AGFAP EAE (Day 23) were hashed before FACS sorting using multiplex 3' Cell Plex Set Kit A (#100261, 10x Genomics). After sorting and washing, astrocytes from 6 samples were pooled and loaded into one well of a 10X Chromium Next GEM Chip G (10X Genomics). Single-cell libraries were further generated using the Single Cell 3' Reagent Kit v3.1 chemistry (10x Genomics) using Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (PN-1000128) according to the manufacturer's protocol. The generated scRNA-seq libraries were sequenced by NOVA-Seq S1 at the Broad Institute.