ONE-STEP tagging: a versatile method for rapid site-specific integration by simultaneous reagent delivery

FCS FILES NAR-20250731T144808Z-1-001.zip - contains raw FACS data (fcs format) grouped by figures Data excel from all figure in paper for statistical analysis_T-TEST_ADDED.xlsx - contains analysis of raw FACS data used in figuresRepresentative_minicircle_ligations_traces_2025-02-11.7z - contains representative minicircle ligation traces from Tapestationmerged_FAY23219_pass.fastq.gz - contains gt-mng-actr10-sorted ONT data (from cells sorted on green fluorescence after tagging with GT mNeonGreen minicircle at ACTR10 locus)merged_FAZ56558_pass.fastq.gz - contains ga-mng-gt-actr10-sorted ONT data (from cells sorted on green fluorescence after tagging with GA mNeonGreen GT plasmid at ACTR10 locus)m84047_250501_103416_s3.hifi_reads.bc2033.bam - contains gt-mng-actr10-sorted PacBio data (from cells sorted on green fluorescence after tagging with GT mNeonGreen minicircle at ACTR10 locus)m84047_250501_103416_s3.hifi_reads.bc2034.bam - contains ga-mng-gt-actr10-sorted PacBio data (from cells sorted on green fluorescence after tagging with GA mNeonGreen GT plasmid at ACTR10 locus)read-classifier-v1.py - script that classifies reads based on alignment scores to different reference sequencesread-coverage-plotter-v1.py - script that plots read coverage per base crispresso_analysis_v1.py - script that analyses and plots indel frequency from crispresso2 output dataMiseq_Tagging_Data.7z - contains Miseq data for attP integration analysisAlleles_frequency_tables.7z - contains crispresso2 output used for attP integration analysisFigure6_images.7z - contains images presented in figure 6EP29012024_Editing and HDR efficiency-20250801T124908Z-1-001.zip - ab1 files, ICE analysis and powerpoint with gel images of attP integrationBenchlingExport_-_2025-08-01.zip - export of benchling folder associated with manuscript (genbank, html and ab1 files)gel_02082024.7z - contains sgd files of gel images, image files and text file with run parameters and legend - figure S4, panel B