Complete dataset from the multiplex TMT-based proteomic experiments.

Complete dataset (unfiltered) from multiplex TMT-based proteomic experiment illustrated in Fig 1A. Normalised, unscaled protein abundances (biological replicates 1–3), log2(ratios), q values, and the numbers of unique peptides used for protein quantitation are shown in the “Complete proteomic dataset” worksheet. Data may be sorted and filtered by log2(ratios) or q values (columns F–K) to identify proteins significantly enriched in EVs (compared with NSCs) or exosomes (compared with NSCs or EVs). q values <0.05 are highlighted in red. In addition, data for any quantified protein of interest may be displayed in the “Gene search and plots” worksheet. Relative abundances (fraction of maximum, mean plus 95% CIs) for each condition are depicted by bars (grey, NSCs; gold, EVs; green, exosomes), as in Fig 1D. q values (highlighted in red if <0.05) and the number of unique peptides used for protein quantitation (with most confidence reserved for proteins with values >1) are also shown. CI, confidence interval; EV, whole EV fraction; Exo, sucrose gradient–purified exosomes; NSC, NSC whole-cell lysates; TMT, Tandem Mass Tag.
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