Comparison of relative mRNA abundance change during purine starvation by SL RNA-seq and qRT-PCR.

qRT-PCR data represents the mean fold change ± standard deviation from two independent biological replicates. ND, not detected in replete or starved sample set; ND* not detected in replete samples only;aserves as an internal control normalized to 1.00 for each qRT-PCR assay;bsince LdNT1.1 peptides were not detected in the 48 h replete sample, the fold change was calculated by comparing the combined average AMT tag intensity recorded at 6, 12, and 24 h (replete samples, n = 11) with the average AMT tag intensity at 48 h (starved samples, n = 5).