Dioscorea dumetorum genome sequence v1.0

The yam species Dioscorea dumetorum is one example of an orphan crop, not traded internationally. Post-harvest hardening starts within 24 hours after harvesting and renders the tubers inedible. Genomic resources are required for trifoliate yam to improve breeding for non-hardening varieties and for other traits. Here, we describe the sequencing of the D. dumetorum genome and the generation of a de novo assembly together with a corresponding annotation. The two haplophases of this highly heterozygous genome are separated to a large extent. The assembly represents 485 Mbp of the genome with an N50 of over 3.2 Mbp. A total of 35,269 protein-encoding gene structures as well as 9,941 non-coding RNA genes were predicted and functional annotations were assigned.

薯蓣属植物薯蓣薯（学名：Dioscorea dumetorum）为一种遗弃作物，在国际市场上并无交易。收获后，其硬实化过程在24小时内开始，使块茎变得不宜食用。针对三叶薯蓣品种，为提升育种对非硬实化品种及其他特性的改良，亟需基因组资源。本研究中，我们描述了薯蓣薯基因组测序过程，以及与之相应的从头组装和注释工作。该高度异源基因组的两个单倍相在很大程度上得到了分离。组装结果代表了基因组中的485兆碱基对，N50长度超过3.2兆碱基对。共预测出35,269个编码蛋白质的基因结构以及9,941个非编码RNA基因，并对其进行了功能注释。