Revisiting DNA barcoding of true bugs of the infraorder Pentatomomorpha (Hemiptera: Heteroptera) from India

Cytochrome c oxidase subunit I gene (COI) sequences of roughly 509 bp length for various species of the Infraorder Pentatomomorpha were generated. K2P divergences within and between species and genera were calculated and compared using newly generated sequences and the ones available on online portals. Mean interspecific (within-genus) genetic divergence (14.23%) was ∼ eight times greater than mean intraspecific (within-species) divergence (1.79%). Distance-based as well as character-based approaches were used towards constructing (COI) trees. In total, 20 sequences were of the species that were previously not part of the Barcode Of Life Database (BOLD), hence representing additions to the barcode library of Indian Heteroptera. Some of the analyzed species are well-known agricultural pests. All the COI sequences and the associated specimen data have been deposited on BOLD.