16S V4-V5 metabarcoding reference databases and weighted naive-bayes classifiers
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下载链接：
https://zenodo.org/record/8301739
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资源简介：
16S metabarcoding databases and naive-bayes classifiers specific to the V4-V5 region. Built from the Silva 138.1 SSU Ref NR 99 database using Qiime2 (version 2023.2 and 2023.5) and the q2-clawback plugin. Includes weighted classifiers for two Earth Microbiome Project Ontology (EMPO) 3 habitat types: "sediment (saline)" and "water (saline)" , with data downloaded from Qiita. Sequences were not dereplicated.
Primers used:
EMP 16S 515f: GTGYCAGCMGCCGCGGTAA
EMP 16S 926r: CCGYCAATTYMTTTRAGTTT
Stats
286,948 unique sequences
388,496 total sequences
46,254 unique taxa (Level 7)
File description
File
Description
make new 16S silva V4-V5 database.md
Markdown with code used to generate databases
silva-138-99-seqs.qza
Full length Silva 138.1 SSU 99 sequences
silva-138-99-tax.qza
Taxa for full length Silva 138.1 SSU 99 database
silva-138_1-99-515f_926r-seqs.qza
Sequences for 16S V4-V5 (primers 515f, 926r), extracted from Silva 138.1 SSU 99, generated by qiime2-2023.2 (forward compatible)
silva-138_1-99-515f_926r-taxa.qza
Taxa for silva-138_1-99-515f_926r-seqs.qza database
uniform-silva-138_1-99-515f_926r-classifier.qza
Unweighted (uniform) naive-bayes classifier for 16S V4-V5 (primers 515f, 926r) extracted from Silva 138.1 SSU 99, generated by qiime2-2023.2 (forward compatible)
silva-138_1-99-515f_926r-q2_2023_2-sediment-saline-classifier.qza
Weighted naive-bayes classifier for 16S V4-V5 (primers 515f, 926r) extracted from Silva 138.1 SSU 99, weighted for sediment-saline, generated by qiime2-2023.2 (forward compatible)
silva-138_1-99-515f_926r-q2_2023_2-sediment-saline-weights.qza
Weights used to generate silva-138_1-99-515f_926r-q2_2023_2-sediment-saline-classifier.qza
silva-138_1-99-515f_926r-q2_2023_5-sediment-saline-classifier.qza
Weighted naive-bayes classifier for 16S V4-V5 (primers 515f, 926r) extracted from Silva 138.1 SSU 99, weighted for sediment-saline, generated by qiime2-2023.5, NOT backwards compatible with older qiime2 versions
silva-138_1-99-515f_926r-water-saline-classifier.qza
Weighted naive-bayes classifier for 16S V4-V5 (primers 515f, 926r) extracted from Silva 138.1 SSU 99, weighted for water-saline, generated by qiime2-2023.2 (forward compatible)
silva-138_1-99-515f_926r-water-saline-weights.qza
Weights used to generate silva-138_1-99-515f_926r-water-saline-classifier.qza
创建时间：
2023-08-31
