Resistance induction against plum pox virus (PPV) by four hairpin constructs in Nicotiana benthamiana

Plum pox virus (PPV) is the most devastating viral disease of the stone fruits worldwide. We studied efficacy of RNA silencing mechanism in induction of resistance against PPV. To do so, N. benthamiana plants were transformed with four different hairpin structures (UTR/P1, HCPro, HCPro/P3 and CP) derived from PPV-T strain. The transgenic plants were challenged by virus inoculation with three different PPV strains and one from each the PPV infected TG plants, PPV infected wild-type (WT) plants, and uninfected (mock) WT plants were subjected to HTS analysis and expression profiles of miRNAs and siRNAs were analyzed to compare efficacy of different hairpin constructs in the induction of transgenic resistance against PPV.