S5 Data -

Fig 8. Wild-type cells outcompete locked-LCD cells in co-culture in minimal medium. (A) Wild-type (marked with Tm) and luxO D61E (marked with Spec) strains of DS40M4 were inoculated at initial frequencies from 1%–99% wild-type (WT) in M9G medium and allowed to grow for 48 h. (B, C) Wild-type (marked with Tm) and luxO D61E (marked with Spec, panel B) or Δqrr1-5 (marked with Spec, panel C) strains of DS40M4 were inoculated at initial frequencies of 80%, 50%, or 20% wild-type (WT) in (B) M9G medium or (C) M9 Casein medium and grown to near stationary phase at t = 16 h, and then diluted to maintain growth in log-phase. Colony forming units (cfu/mL) were measured at each time point using selective antibiotics for each strain. The total population number was calculated, and the data are represented as the percent of WT cells in the total population of WT and luxO D61E or WT and Δqrr1-5. The data are representative of 3 biological experiments.
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