Compilation of transcriptomics, non-targeted metabolomics and targeted metabolomics data in bovine mammary epithelial cells stimulated by ten different essential amino acids (Untargeted metabolomics)

Divergence in the non-targeted metabolite profilings of bovine mammary epithelial cells (BMECs) were systematically captured with regard to 10 individual essential amino acid (EAA) availability. BMECs cultured in 6-well plates were first serum-starved overnight and subsequently assigned to 1 of 12 treatment media (n = 6). DMEM-F12 medium is the positive control treatment (POS), while DMEM-F12 medium devoid of all EAA served as the negative control treatment (NEG). A total of 10 treatments were NEG individually supplemented with arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan or valine (Sigma-Aldrich, MO, USA). Individual EAA were supplemented to achieve concentrations equal to those of POS. After 6-h treatment, all cell samples were taken at the same time, frozen in liquid nitrogen and stored at -80 °C until required.

Untargeted metabolomics is reported in the current study MTBLS7789.
Targeted metabolomics is reported in MTBLS3956.