SRCAP Mutations Drive Clonal Hematopoiesis Through Epigenetic and DNA Repair Dysregulation [Cut & Run]

To investigate how Srcap mutations confer mutant hematopoietic stem and progenitor cells (HSPCs) with selective advantage after transplantation We assessed SRCAP chromatin remodeling and corresponding H2A.Z incorporation using Cut-and-run sequencing Overall design: We established Srcap heterozygous mutant murine competitive transplantation model. Donor test (CD45.2+) HSPCs in the bone marrow were sored and isolated at 12 weeks post-transplantation for Cut-and-run sequencing