NMuMG cell response to TGF-beta and TGF-beta +2APB treatments

Recent studies demonstrate that Ca2+ signaling has an important role in EMT. Use of Ca2+ blockers such as 2APB can inhibit cell migration induced by TGF-ß. Interestingly, we see an unexpected increase in Snail expression upon Ca2+ blocker treatment of both MCF10A and NMuMG cells; this increase is not observed with 2APB treatment alone. Therefore, we believe that 2APB plays a synergistic role with TGF-ß in Snail induction. We propose to investigate the gene networks that change following 2APB +TGF-ß treatment. Overall design: mRNA profiles of NMuMG cells treated with TGFbeta, TGFBeta and 2APB, and DMSO control were generated using deep sequencing, in duplicate