Intercalibration of the Estimated Oil Equivalence (EOE) of WAFs and CEWAFs using 3 different spectrofluorometers

This dataset contains the results of three intercalibration experiments using three different spectrofluorometers (Horiba Aqualog-UV-800, Turner Trilogy, and Shimadzu Spectrofluorometer RF-1501) to measure the concentration of oil in blind samples of Water Accommodated Fraction (WAFs) and Chemical Enhanced WAF (CEWAFs). The first experiment was designed to analyze a set of WAF samples from mesocosms (Wade et al. 2017) using the three different fluorometers. Water samples were measured directly (i.e., no extraction process was involved). The second set of measurements were done on the same source water samples but this time, samples were extracted with dichloromethane. The fluorescence emissions from the three instruments were recorded and compared. The second experiment consisted of the intercalibration between the Aqualog Horiba and Turner Trilogy spectrofluorometers. For this, WAF and CEWAF samples were made in deionized water (DI) as well as in natural seawater collected from the Gulf of Mexico immediately prior to starting experiments. The chemical response to oil spills: Ecological Research Forum (CROSERF) method was used to make the WAF and CEWAF (Singer et al., 2000). The third experiment consisted of the Intercalibration between Aqualog Horiba and Shimadzu spectrofluorometers. WAF and CEWAF samples from ADDOMEx 6 mesocosms study were used. WAF and CEWAF were made following Wade et al., (2017) method.