Gene expression pattern comparison depend on morphological change in rat pXEN cells

pXEN cells have intrinsic property for vacuolation generally and in proper condition, vacuolated cells contribute to cavitation, so call vesiculation, via merge together. So, we want to find meaning of morphogenesis and connection with in vivo gastrulation stage. Overall design: Samples were cultured in each different condition and prepared each 3 per sample independently. Vacuolated cells were collected from three differnet way, in BCS and Chir99021 with and without sorting, and vesicles were prepared from two condition, with and without serum.