Genome-nuclear lamina interactions in the heart at embryonic days E9.5 and E12.5

Lamin B1 ChIP-seq analysis of microdissected whole hearts from E9.5 and E12.5 CD1 embryos. Results provide insight into in vivo genome-nuclear lamina interactions over the course of embryonic development. Progenitor cells require coordinated expression of lineage-specific genes to regulate differentiation into daughter cell types. Epigenetic regulators are ideally positioned to synchronize transcription of disparate genomic loci. Using cardiac development to model progenitor cell behavior, we map genome- lamina interactions in vivo to reveal dynamic changes during cardiac development and propose that Hdac3 mediates chromatin organization and gene positioning in cardiac progenitors to regulate fate decisions. Overall design: Examination of Lamin B1 occupancy in embryonic hearts at 2 different time points. 4 biological replicates at E9.5, 3 biological replicates at E12.5.