Effect of embryo lysates treatment on gene expression.

To systematically evaluate the effect of embryo lysates feeding, we conducted RNA sequencing (RNA-seq) on day 7 N2 worms treated with embryo lysates. Our findings revealed that embryo lysates affected the expression of genes related to fatty acid metabolism, degradation of branched-chain amino acids, and lysosome functions. Notably, worms treated with embryo lysates showed increased fatty acid degradation gene expression and decreased fatty acid elongation gene expression. Overall design: Day 7 N2 worms were treated with embryo lysates for 24h. Then worms were collected for mRNA seq and gene expression profiliing analysis.