the profiles of m6A modification in villous tissues with embryo damage

Three pairs of villous tissue of embryo damage samples and painless abortion samples were obtained from Shenzhen Second People's Hospital. The villus tissue of patients was collected immediately after fetal discharge, rinsed with precooled normal saline three times, and the broken tissue with a diameter of 1mm was cut with ophthalmic scissors. After the tissue was mixed with Trizol solution in a volume ratio of 1:10, it was ground to homogenate with a freeze grinder, and the left tissue was frozen with liquid nitrogen.