The Protein Elicitor Hrip1 Enhances Resistance to Insect and Ahead of Time of Flower Bolting in Arabidopsis thaliana

The elicitor Hrip1 isolated from necrotrophic fungus Alternaria tenuissima, could induce systemic acquired resistance in tobacco to enhance resistance to tobacco mosaic virus. In the present study, we found that Hrip1 was overexpressed in wild type (WT) Arabidopsis thaliana. Our results indicate that the transgenic lines were more resistant to Spodoptera exigua and ahead of time of flower bolting than the WT line. A profiling of transcription assay using digital gene expression profiling was used for transgenic and WT Arabidopsis thaliana. Differentially expressed genes including 40 upregulated and three downregulated genes were identified. In transgenic lines of Hrip1 overexpression, three genes related to Jasmonate (JA) biosynthesis were significantly upregulated, and the JA level was found to be higher than WT. Two GDSL family members (GLIP1 and GLIP4) and pathogen-related gene, which participated in pathogen defense action, were upregulated in the transgenic line of Hrip1 overexpression. Thus, Hrip1 is involved in affecting the flower bolting time and regulating endogenous JA biosynthesis and regulatory network to enhance resistance to larvae.