Gene expression profile of HMT3522 S1 cells grown on 2D and in 3D matrix in response to the death ligand TRAIL

The series represent gene expression profiles of HMT3522 S1 mammary epithelial cells cultured as 2D monolayers or in 3D reconstituted basement membrane (rBM) and treated with the death ligand TRAIL. Keywords: Genetic modification; response to death induction Three replicated cultures were established for each of the 4 experiment groups, including S1 cells grown as 2D monolayers (S1-2D CA, S1-2D CB, S1-2D CC), S1 cells grown as 2D monolayers and treated with TRAIL (S1-2D TA, S1-2D TB, S1-2D TC), S1 cells embedded in 3D rBM (S1-3D CA, S1-3D CB, S1-3D CC), S1 cells embedded in 3D rBM and treated with TRAIL (S1-3D TA, S1-3D TB, S1-3D TC). Cells were embeded in rBM for 12 days and then treated with TRAIL (1 μg/mL) or vehicle for 8 hours before the collection of RNA samples.