IL-6 driven pSTAT1 response is linked to T cell features implicated in early immune dysregulation

Here, we performed an in-depth characterization of the effect of classic IL-6 signaling in primary human T cells from healthy individuals. We generated a detailed map of IL-6 signaling in CD4+ and CD8+ naïve and memory T cell subsets, linking mbIL-6R expression levels to the magnitude and balance of phosphorylation of STAT1 and STAT3 and the transcriptomics response to IL-6 in these subsets. Overall design: Primary human CD3+ T cells derived from healthy donors with the rs2228145 AA genotype were cultured with or without IL-6 (20 ng/mL) for 4 hours and the transcriptomic response to IL-6 signaling was determined in flow sorted T cell subsets including CD4+ naïve (CD4+CD45RA+), CD4+ memory (CD4+CD45RO+) and CD8+ naive (CD8+CD45RA+) T cells.