Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells. Mus musculus

ZFAT is a nuclear protein that harbors putative DNA-binding domains. Studies in mice have shown that ZFAT plays a critical role in survival and differentiation in T cells. However, molecular mechanisms whereby ZFAT regulates gene expression programs in T cells remain poorly unknown. We here identify the direct target genes of ZFAT in thymocytes through combination of chromatin immunoprecipitation with sequencing and transcriptional profiling. Overall design: ChIPseq data were obtained for a zinc-finger transcription factor, ZFAT, and histone modifications (H3K9me3 and H3K9ac/K27ac) to further elucidate the functions of ZFAT in thymocytes.