Transcriptome profiling Zebrafish Sap130b gene mutations in embryogenesis

This study profiled the transcriptome of zebrafish embryos harboring mutation in the Sin3A associated Protein 130b (sap130b) gene at 36 hours post fertilization (hpf). The sap130b mutant was generated via Cas9 mediated gene editing. Zebrafish sap130b mutants are viable and survive as adults with no observable phenotype. Maternal Zygotic sap130b mutants were established and they were also normal and it was reasoned that sap130a was compensating for the loss of sap130b. A transcriptome analysis was performed at 36 hpf to compare with our analysis of sap130a at this stage. Total RNA was extracted from embryos at 36 hours post fertilization from wildetype, MZsap130b mutants.