Virus could not be recovered when all PKs are deleted.

Genomes of FMDV wt and modified FMDV sub-genomic replicons were converted into infectious viruses by swapping the GFP reporter gene with sequence encoding capsid of O1K FMDV infectious copy clone. RNA transcribed from these wt or modified FMDV genome clones was transfected into BHK-21 cells and appearance of CPE observed over 5 sequential passages. At the 4th passage virus was harvested and sequenced to observe for any changes within the sequence. Presence of CPE indicated with ‘Y’ while ‘N’ represents no CPE seen.