Validation of the anti-Ro60 antibody (sc-100844, Sants Cruz Biotechnology, Inc) by coimmunoprecipitation from total HEK293 cell lysates

The mouse monoclonal anti-Ro60 antibody (sc-100844, Sants Cruz Biotechnology, Inc) has been validated by coimmunoprecipitation from total Flp-In T-REx 293 cell lysates. An anti-His antibody (SAB1305538, Sigma-Aldrich) was used as a negative control (see experimental details in Jeandard et al., "CoLoC-seq probes the global topology of organelle transcriptomes", manuscript in preparation). Ro60 was found to be the most strongly enriched protein (~23-fold enrichment). Low-level co-enrichment of other proteins (including mitochondria-localised ones) has also been observed. These data validate the specificity of the anti-Ro60 antibody.