Innate immune responses in the alveolar space to different formulations of SDI RNA

The aim of this experiment was to analyse the innate immune response in alveolar cells after different nanoparticle formulations are delivered intranasally in 50 µl PBS with or without 1 µg Sendai virus Defective Interfering RNA Overall design: Naive BALB/c mice or mice exposed to influenza B 4 weeks before treatment are given 1 µg of SDI RNA formulated in different nanoparticles intranasally. 4h later, lung alveolar cells are harvested by bronchioalveolar lavage and RNA is extracted for transcriptomic analysis.