Bacillus velezensis strain:5RB Genome sequencing and assembly

The whole genome sequence (WGS) of a novel isolate Bacillus sp. strain 5RB was completed. WGS was based on TruSeq DNA PCR-library, Illumina SBS technology and Prokka pipeline identifying the locations of protein-coding sequences, tRNA, and rRNA genes (Macrogen Inc.). The genome of Bacillus sp. strain 5RB is 3.91 Mbp in size, without plasmids, and contains 3839 genes. The largest part of the genome is connected with amino acid transport and metabolism (289 genes), carbohydrate transport and metabolism (225), inorganic ion transport (176), and energy production and conversion (170 genes). Cell wall, the membrane, and envelope were encoded by 176 genes; translation, ribosomal structure, and biogenesis - by 161; post-translational modifications and chaperones - by 96 genes. Interestingly, 871 genes are with unknown functions (23% of the genome). Considering the species affiliation of the novel strain, the sequences of 5S, 16S and 23S rRNAs showed that Bacillus sp. 5RB is a member of B. amyloliquefaciens group, closely related to B. velezensis, B. siamensis and B. tequilensis.