Analysis of NFκB binding sites in the apoptosis genes.

The promoter regions of BAX and BCL2 from −5000 to +2000 relative to the transcription start site were analyzed for the occurrence of NFκB binding sites using the Opossum web-service. The identity of the sequence used for analysis (ENSEMBL ID), the chromosome number (Chr.), the actual transcription factor binding site, the position relative to the transcription start site and the orientation are indicated. The Z-score uses the normal approximation to the binomial distribution to compare the rate of occurrence of a TFBS in the target set of genes to the expected rate estimated from the pre-computed background set. The likelihood that the number of TFBS nucleotides detected for the included target genes was significant as compared with the number of TFBS nucleotides detected for the background set. Z-score was expressed in units of magnitude of the standard deviation.