Effect of oxidized lipid concentration on electropore formation time in oxPLPC:PLPC bilayers.

Bilayer thickness (distance between the two mean phosphorus planes) and area per lipid are mean values taken from three independent simulations for each condition over 100 ps time steps from the beginning of the simulation until 1 ns before pore formation. For systems where poration occurred in less than 1.5 ns, the cutoff is 0.5 ns before pore formation. Bilayer thickness values from independent simulations vary by less than 10%. Area per lipid is the area of the bilayer divided by the number of lipids in one leaflet of the bilayer (36). Individual and mean times to poration are shown for three independent simulations of each system. Systems contain 2880 water molecules and 72 total lipids. The applied electric field is 360 mV/nm.