Arabidopsis thaliana Raw sequence reads

Five-day-old seedlings grown under white light were treated with or without low R/FR (0.8) or very low R/FR (0.2) for 1 hr, and whole seedlings were collected. RNA-sequencing were conducted using a BGISEQ500 platform. Differentially expressed genes screening by NOISeq method. We find that shade induction of auxin response genes expression by very low R/FR (0.2) significantly decreased than that by low R/FR (0.8) in wide type, and therefore reflect the correlation between auxin and shade-induced hypocotyl elongation. Moreover, with 1hr low R/FR (0.8) treatment, there is no significant difference of shade induction between the two genotype, but the shade inductions of auxin responsive genes expression is significant enhanced in phyA mutant than that in Col-0 after 1 hr very low R/FR (0.2) treatment. Taken together, these data indicated the enhanced auxin response in phyA-211 under very low shade condition.