Low input RNA Sequencing of effector and naive WT and NCOR1-cKO CD4+ Treg cells

Analysis of Treg cells isolated from WT and NCOR1-cKO mice indicated an important function of NCOR1 in regulating the transition from naïve to effector Treg cells. The aim of this NGS experiment was to determine the transcriptome profile of ex vivo isolated naïve and effector WT and NCOR1-cKO Treg cells under steady state condition. mRNA expression profiles of naïve (CD44loCD62L+) and effector (CD44hiCD62L-) WT and NCOR1-cKO Treg cells were generated by low input RNA Sequencing. Therefore, Treg cells were isolated from the spleen and lymph nodes of either WT mice or mice with a conditional deletion of NCOR1 (using Ncor1fl/fl Cd4-Cre mice). The mice additionally harbored a DEREG Foxp3-eGFP reporter, in which GFP expression is driven by Fopx3 regulatory elements (Lahl et a. 2007). Cells were FACS sorted using a SH800 Sorter (SONY).