Effect of smooth muscle specific PRDM6 depletion on Aorta and Ductus Arterious (DA) development

To investigate the function of PRDM6 in smooth muscle cells during outflow tract development, we have isolated the ascending aorta and the ductus arteriosus (DA) separately from the E18.5 Wnt1Cre;PRDM6f/f mouse (PRDM6 KO) and PRDM6f/f mouse (Control) and compared the gene expression profile of the four groups of samples There are four groups of samples in our experiment (Control Aorta; PRDM6 KO Aorta; Control DA; PRDM6 KO DA), and we focused on comparing Control DA v.s PRDM6 KO DA为探究PRDM6在流出道发育过程中平滑肌细胞内的功能，我们分别从E18.5天的Wnt1Cre;PRDM6f/f小鼠（PRDM6敲除组，PRDM6 KO）与PRDM6f/f对照小鼠（Control）中分离升主动脉与动脉导管（ductus arteriosus, DA），并对四组样本的基因表达谱进行比较分析。本实验共设置四组样本：对照组升主动脉（Control Aorta）、PRDM6敲除组升主动脉（PRDM6 KO Aorta）、对照组动脉导管（Control DA）以及PRDM6敲除组动脉导管（PRDM6 KO DA），我们的研究重点为对照组动脉导管相较于PRDM6敲除组动脉导管的差异分析。