Gene expression in different root types in pearl millet (Pennisetum glaucum (L.) R. Br)

Pearl millet (Pennisetum glaucum) is a cereal crop well-adapted to arid and high-temperature environments. Its root system exhibits a high degree of complexity, comprising distinct root types with specific anatomical and functional characteristics. These include the primary root, which is established during embryogenesis; crown roots, which develop at the base of the stem post-embryonically; and lateral roots, which emerge from both the primary and crown roots. In this study, we conducted a comparative analysis of gene expression profiles across these distinct root types to better understand their functional specialization. Overall design: Pearl millet plants of the Tift23DB genotype were grown for 15 days in rhizotrons as described (Passot et al., 2018). Different root types (primary root, crown and lateral roots) were then sampled and used for RNA extraction and sequencing.